September 2013 Vol. 1 No. 1

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Full Length Research Paper

The antioxidant effect of picrosideⅡand the optimizing of therapeutic dose and time window in cerebral ischemic injury in rats

Meizeng Zhang¹*, Pang Fangfang¹, Rui Zhang², Li Zhao¹ and Yiling Wu³

¹Department of Neurology, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China
²Department of ICU, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China
³Department of Rehabilitation, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China

*Corresponding Author's E-mail: zmzcmd@163.com; Tel: +8618661808039

Accepted August 26, 2013

Abstract

To optimize the antioxidant effect and the best therapeutic dose and time window of picrosideⅡin cerebral ischemic injury in rats by orthogonal test. The forebrain ischemia models were established by bilateral common carotid artery occlusion (BCCAO) methods. The successful rat models were randomly divided into 16 groups according to orthogonal experimental design of [L₁₆(4⁵)] and treated by injecting picrosideⅡintraperitoneally with different doses at various times. The concentrations of malondialdehyde (MDA), nitric oxide (NO) and hydrogen per oxide (H₂O₂) in serum and brain tissue were respectively determined by thiobarbituric acid assay, nitratase reductase assay and chemiluminescence’s immunoassay. The optimized compositions of the therapeutic dose and time window of picrosideⅡin cerebral ischemic injury were (1) ischemia 1.5h with 10mg/kg, 1.5h with 20mg/kg and 1.5h with 10mg/kg body weight according to the expressions of MDA, NO and H₂O₂ in serum respectively, (2) ischemia 1.5h with 10mg/kg, 1.5h with 20mg/kg and 1.5h with 20mg/kg body weight according to the expressions of MDA, NO and H₂O₂ in brain tissue respectively. Conclusion On the basis of the principle of the minimization of medication dose and maximization of therapeutic time window, the optimized composition of the therapeutic dose and time window in cerebral ischemic injury was injecting picroside Ⅱintraperitoneally with 10-20mg/kg body weight at ischemia 1.5h.

Keywords: picroside Ⅱ, cerebral ischemia, therapeutic dose, time window, MDA, NO, H₂O₂, rats