January 2016 Vol. 4 No.1

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Original Research Article

Determination of Micro RNA 126/126* and Their Target Gene Epidermal Growth Factor Like-7 Expression Profiles in Turkish Breast Cancer Patients

Çağrı ÖNER^1*, Didem TURGUT COŞAN¹, Mustafa Serdar ERKASAP², Evrim BAYMAN³, Hasan Veysi GUNE޹, İrfan DEĞİRMENCݹ and Fezan MUTLU ŞAHİN⁴

¹Eskisehir OsmangaziUniversity, Medical Faculty, Department of Medical Biology, ESKİSEHİR/TURKEY
²Eskisehir Osmangazi University, Medical Faculty, Department of General Surgery, ESKİSEHİR/TURKEY
³Eskisehir Osmangazi University, Medical Faculty, Department of Radiation Oncology, ESKİSEHİR/TURKEY
⁴Eskisehir Osmangazi University, Medical Faculty, Department of Biostatistics and Medical Informatics, ESKİSEHİR/TURKEY

*Corresponding Author’s E-mail: coner86@gmail.com

Accepted January 02, 2016

Abstract

Micro RNA-126 (miR-126) and its target gene, Egfl-7, are known to enhance apoptotic processes and also play a role in vascular growth through the regulation of vascular endothelial growth factor (VEGF)-mediated signalling, angiogenesis, and vascular integrity. In this study, we aimed to evaluate differences in the expression levels of miR-126, its complementary miR-126* and Egfl-7 in tumour specimens and peripheral blood mononuclear cells (PBMC) of breast cancer and metastasis. The samples used in this study were provided by the Eskisehir Osmangazi University, Medical Faculty, the Departments of General Surgery and Radiation Oncology. Real Time Polymerase Chain Reaction (RT-PCR) was used to determine the gene expression levels in PBMC and breast cancer tumours. Our study showed that miR-126 and miR-126* expressions in breast cancer are significantly decreased relative to those in PBMC in healthy controls (P < 0.001) and breast cancer tumour specimens (P ≤ 0.001). Furthermore, a statistically significant increase in Egfl-7 gene expression was observed in the PBMC of breast cancer and metastasis (P ≤ 0.001) and in breast cancer tumour specimens (P ≤ 0.001). The results from the PBMC and tumour samples suggest that miR-126 and Egfl-7 may impact the development and metastasis of breast cancer.

Key Words: Breast Cancer, Egfl-7, Metastasis, microRNA-126, microRNA-126*