September 2015 Vol. 3 No. 9

Search Pubmed for articles by:
 

Original Research Article

Synergistic effects of the bismuth nanoparticles along with antibiotics on PKS positive Klebsiella pneumoniae isolates from colorectal cancer patients; comparison with quinolone antibiotics

Zahra Tarjoman¹, Shahla Mohammad Ganji²* and Sedigheh Mehrabian¹

¹Department of Microbilogy, Faculty of Life Sciences, Azad Islamic University, North Tehran branch, Tehran, Iran
²National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

*Corresponding Author’s Email: shahlamg@yahoo.com
Telephone: (+98) 21 44787466
Fax: (+98) 21 44787399

Accepted July 06, 2015

Abstract

Colorectal cancers (CRC) are among the most prevalent types of cancers in the world and in Iran, wherein, the age of affliction is lower than Western countries. We studied the antimicrobial effect of the bismuth nanoparticles on Klebsiella pneumoniae carrying PKS gene. PKS encodes colibactin which makes colorectal cancers more probable. In the present study we identified and isolated the PKS carrier isolates through application of PCR. Subsequently, the antibacterial effects of the bismuth nanoparticles, along with antibiotics including tetracycline; ciprofloxacin, Norofloxacin, and metronidazole were investigated on bacterial isolates. Results show all isolates were sensitive to all applied antibiotics, however, they showed resistance to the bismuth nanoparticles. Analysis on the synergistic effect of the bismuth nanoparticles with the aforementioned antibiotics shows a synergistic effect, however, there was difference between bacterial isolates as well as the degree of synergism. Results obtained in this study indicate, compared to the antibiotics, the bismuth nanoparticles could be effective against digestive system’s infection to lower extents. As well, it could be effective in lowering the colorectal cancer in the individuals’ carrier for the isolates.

Keywords: Bismuth naoparticles, colibactin, colorectal cancer, Klebsiella pneumoniae, PKS